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COVID-19 (N501Y)

Ngwa PCR ozugbo maka akwụkwọ akụkọ Coronavirus 2019-nCoV

Ịghọta nchoputa dị iche na ụdị anụ ọhịa na ụdị mutant (501Y).


Nkọwa ngwaahịa

Mkpado ngwaahịa

ndabere

Ngalaba na-anata ihe nnabata Spike glycoprotein (RBD) nke SARS-CoV-2 na-emekọrịta njikọ nke nje virus na ihe nnabata angiotensin na-agbanwe enzyme 2 (ACE2) n'elu sel mmadụ.Ya mere, mmekọrịta Spike-ACE2 bụ ihe dị mkpa na-ekpebi ihe kpatara nje nje virus. Otu ọhụrụ phylogenetic nke SARS-CoV-2 (usoro ọmụmụ B.1.1.7), nke na-egosipụta nnọchi amino acid na Spike RBD (N501Y mutation).

Dabere na nyocha ọhụrụ sitere na Ngalaba Ahụike UK, dabere na data nke usoro genome nke usoro, ọrịa na-efe efe na ụdị, ọrịa nke ụdị dị iche iche nke SARS-CoV-2 fọrọ nke nta ka ọ bụrụ 40-70% dị elu karịa ndị ọkà mmụta sayensị mgbanwe mgbanwe ndị ọzọ ghọtara. .Nzaghachi na nchọpụta ngwa ngwa bụ otu n'ime ihe mgbochi na njikwa dị mkpa.

Atụmatụ

izi ezi

Ngwa ahụ na-anabata usoro ARMS, Ghọta uche nke nchọpụta mmụgharị isi;

Njikwa endogenous ( gene RdRp) gụnyere maka mmịpụta nlele;

Nkọwapụta

Na-egbochi mmetọ site na iji UDG (Uracil-DNA Glycosylase System);

Mmetụta: 200 mbipụta / ml;

arụmọrụ

Kachasị mma reagent usoro, mfe components, na-azọpụta oge nchọpụta;

Dakọtara na ọtụtụ ụdị ngwa PCR;

Kwesịrị ntụkwasị obi

Nsonaazụ na-emegharị ugboro ugboro ma na-emegharị;

Ọ dịghị cross-reactivity na ndị ọzọ iku ume nje;

Ụkpụrụ

A na-eji ngwa ahụ maka nyocha qualitative in vitro ịchọpụta mkpụrụ ndụ ihe nketa ORF1ab/N/S nke novel coronavirus 2019-nCoV n'ụdị akụkụ iku ume, na ịme mmụgharị mkpụrụ akwụkwọ na N501Y n'otu oge.

Ngwa ahụ na-anabata usoro ARMS, were S gene of novel coronavirus (2019-nCoV) dị ka mpaghara ebumnuche maka mmụgharị mkpụrụ akwụkwọ, na-echepụta primers na nyocha maka N501Y.

Ngwaahịa

Nkọwapụta

Chekwa okpomọkụ

Nkọwa

Ngwa PCR Real Time maka akwụkwọ akụkọCoronavirus 2019-nCoV naAkwụkwọ akụkọ Coronavirus 2019-nCoVMgbanwe mkpụrụ ndụ ihe nketa (N501Y) 48T -20 ℃ Nyocha: Ụdị anụ ọhịa nke N501Y,Ụdị mgbanwe nke N501Y, ORF1ab,N gene na njikwa ime

Ndakọrịta template

  • Oropharyngeal swabs
  • Nasopharyngeal swab
  • Mmiri Lavage Bronchoalveolar
  • Sputum

Ngwa ngwa

  • Ihe nchekwa mmeghachi omume RT-PCR
  • Ngwakọta enzyme RT-PCR
  • Ụdị anụ ọhịa dị mma njikwa
  • Mgbanwe nke ọma njikwa
  • Njikwa adịghị mma

Nsonaazụ ule

 

 

Ụdị Mgbanwe N501Y

Ụdị Mgbanwe N501Y

Ụdị anụ ọhịa N501Y

Ụdị anụ ọhịa N501Y


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