A na-eji ngwa ahụ maka ịchọpụta mkpụrụ ndụ ihe nketa ORF1ab/N/S nke coronavirus 2019-nCoV n'ụdị akụkụ iku ume gụnyere swabs oropharyngeal, nasopharyngeal swab, sputum na ime mmụgharị mkpụrụ ndụ na HV69-70 del n'otu oge.Dị ka a kọrọ na GISAID nchekwa data, ihe karịrị 95% Omicron variant usoro kọrọ gụnyere ihichapụ na HV69-70, nke nwere ike ime ka S gene target failure (SGTF) na PCR assays.Enwere ike iji SGTF dị ka ihe nrịbama proxy iji lelee Omicron.
Emebere usoro izizi na nyocha FAM maka nchọpụta kpọmkwem nke ORF lab gene nke 2019-nCoV, VIC akara nyocha maka N gene nke 2019-nCoV, ROX akara nyocha maka S gene HV69-70 del mutation nke 2019-nCoV.Mkpụrụ ndụ RNase P nke mmadụ ewepụtara n'otu oge yana nlele ule na-enye njikwa dị n'ime iji kwado usoro mmịpụta nucleic na iguzosi ike n'ezi ihe.Nchọpụta ezubere iche maka mkpụrụ ndụ ihe nketa RNase P mmadụ ka akpọrọ CY5.
Sistemụ PCR dị adị: Aehealth Diagenex AL, ABI 7500, ViiATM 7, Quant Studio 7 flex.Roche Lightcycler 480, Agilent Mx3000P/3005P, Rotor-GeneTM6000/0.Bio-Rad CEX96 TouchTM SLAN-96S.SLAN-96P, MA 6000;